A direct injection of various culture supernatants into a column of Porapak Q was investigated as a rapid and simple technique for gas chromatographic analysis of volatile metabolites produced by microorganisms. The usual metabolites such as methanol, ethanol, formic acid, 2‐propanol (or acetone), 1‐propanol, acetic acid, diacetyl, 1‐butanol, propionic acid, acetoin, butyric acid, 2,3‐butanediol and lactic acid were detected without any pretreatment. It was demonstrated that most strains of Bifidobacterium bifidum (Lactobacillus bifidus) produced both acetic acid and lactic acid which contrasts with results from Escherichia coli and other Lactobacilli.
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Yoshioka et al. (1969) studied this question.
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