Microchemical Studies on the Golgi Apparatus Using Protease Nile Blue Sulphate Technique II

The contour of the Golgi apparatus of the pancreatic acinar cells in white mouse is gradually augmented in thickness as the degree of osmium impregnation increases.2. The Golgi apparatus of the acinar cell was disclosed by the writer's own new proteolytic enzyme-Nile blue sulphate technique. The Golgi apparatus thus prepared accords fully with that impregnated properly after Kolatschev's method. This fact means that the classical metallic impregnation methods when employed properly give an exact result at least in the fixed material.3. By the application of the new technique it becomes evident that the zymogen granules contain more or less quantity of a certain lipoid substance.4. Photomicrographs of the Golgi apparatus in the living acinar cells could be taken successfully on the preparations stained by the intraperitoneal injection of Nile blue sulphate. The Golgi apparatus are stained light blue assuming irregular networks, which are quite similar to those demonstrated by means of the writer's new technique and the classical osmium impregnation methods when properly applied. It is verified eventually that the Golgi apparatus really exists in the living cells with the same morphology as revealed by the classical technique.5. In the living acinar cells the vacuomes, which stain intensely blue with Nile blue sulphate, are easily distinguishable from the genuine Golgi apparatus.6. The cytoplasm of the acinar cells is not digestible with pepsin, while trypsin digests the cytoplasm and the protein component of the Golgi apparatus. The lipoid substance of the Golgi apparatus remaining after the trypsin digestion is insoluble in aceton and is easily dissolved in alcohol. This property of solubility means that the lipoid component of the Golgi apparatus of the acinar cells is a lecithin-like substance, which is not contained in the apparatus of hepatic cells of the same animal and the newt. This difference in chemical nature leads the writer to suppose manifold modifications in the ingredients of the apparatus as the tissue differs.7. Judging from the nature of solubility in the organic solvents and in the enzyme, the lipoid substance located on the cell boundaries seems to be the same substance which is contained in the Golgi apparatus.