Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana

The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana . The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analyses support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Implications of this model for ethylene signaling are discussed. Background: Plant responses to the hormone ethylene depend on ethylene receptors and the kinase CTR1. Results: The receptors and CTR1 exist as signaling complexes whose levels change in response to ethylene. Conclusion: A model incorporating transcriptional induction and ethylene-dependent turnover of receptor/CTR1 complexes is proposed. Significance: Results presented here reconcile molecular responses at the receptor level with physiological changes in sensitivity to ethylene.