Immunohistochemical Analysis and Pathological Assessment of B-Cbl Proto-Oncogene in Gastric Carcinoma Cells

In order to study the status and expression of Cbl-b oncoprotein in gastric carcinoma we underwent an immunohistochemical protocol by which we analyzed the immuno-distribution as well as the level of Cbl-b expression on gastric cancerous tissues. Material and MethodsNinety-six (96) paraffin blocks of cancerous gastric tissues sections were collected from the Department of pathology of Oran Military Hospital (Western Algeria) for the period of 2006-2015.All the cases were identified histologically as malignant gastric cells.These specimens were paraffin embedded in the same center.The cases were stained with hemotoxyline and eosin (H&E) for routine histological examination.An absolute confidentiality of the patients' vital information was maintained for ethical purposes and an ethical approval was obtained from the institution in which the study was carried out. Immunohistochemical protocolWe used the following Abcam protocol of immunohistochemistry (IHC): formalin-fixed paraffin-embedded tissue sections were deparaffinized and rehydrated then enough drops of Hydrogen Peroxide Block were added to cover the sections, they were incubated for 10 minutes then washed twice in buffer Phosphate Buffered Saline (PBS) (10X PBS) (0.1M PBS, pH 7.4).A pretreatment was performed then the sections were washed 3 times in buffer.