Nutritional Studies on the Chloromonadophyceae: Vacuolaria Virescens and Gonyostomum Semen12

SUMMARY Unialgal, nonaxenic cultures of Vacuolaria virestcens Cienkowsky were grown in soil‐water medium, in simple salt solutions with added peat and soil extract, and in synthetic medium. Doubling times in different media were determined: maximum growth rate (doubling time = 46.0 hr) was obtained in continuous light (210 ft‐c) at 24 ± I C. Gonyostomum semen (Ehrenberg) Diesing was isolated from a natural population and grown in soil‐water medium and a synthetic medium. Other techniques that have been used for culturing chloromonadophycean algae are reviewed.