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Laboratory experiment demonstrates binding of bacterial mucopolysaccharidases to glycosaminoglycan-coated matrices, indicating the importance of matrix pre-coating.
Chondroitinase C, chondroitinase AC, heparinase, and heparitinase separated from an extract of Flavobacterium heparinum were subjected to affinity chromatography with glycosaminoglycan-bound AH-Sepharose 4B, previously coated non-covalently with glycosaminoglycan, as the matrix. The results suggested the importance of coating the matrix with glycosaminoglycan in the binding of the enzyme protein to the matrix.
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Ototani et al. (1978) studied this question.
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