Recently, alternative assays for FFA, based on specific enzymic oxidation by acyl-coenzyme-A-synthetase (ACS) and acyl-coenzyme-A-oxidase (ACOD), have been developed (Shimizu et al. [2]). Hydrogen peroxide generated by these reactions can be quantitated colorimetrically (Mizuno et al. [3]; Shimizu et al. [4]; Matsubara et al. [5]
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Knox et al. (1984) studied this question.