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September 1, 1969Proceedings of the National Academy of SciencesOpen Access

A Genetic Assay for Mrna's of Phage T4

R. Jayaraman Edward B. Goldberg

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Abstract

A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene gammaIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.

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Cite This Study

Jayaraman et al. (1969) studied this question.

synapsesocial.com/papers/6a911f6802b8c0248acaee46 https://doi.org/10.1073/pnas.64.1.198

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