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November 1, 1972Proceedings of the National Academy of SciencesOpen Access

Blocking by Histones of Accessibility to DNA in Chromatin: Addition of Histones

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Authors

AMA. E. MirskyGates (United States)BSB. D. SilvermanIBM (United States)NPNimai C. Panda

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Implication

Biochemical assay demonstrates that lysine-rich histones restrict DNase access to DNA more effectively than arginine-rich histones, indicating that phosphorylation modulates chromatin compaction.

Key Points

  • To assess how different histone fractions and phosphorylation modulate the accessibility of DNA to DNase in intact and histone-depleted chromatin.
  • Added specific histone fractions to chromatin within intact thymus nuclei, histone-depleted nuclei (depleted of lysine-rich or arginine-rich histones), and extracted deoxyribonucleoprotein.
  • Measured DNA accessibility through enzymatic digestion using DNase.
  • Lysine-rich histones blocked DNA accessibility to DNase substantially more effectively weight-for-weight than arginine-rich histones across all nuclear and chromatin preparations.
  • Subfractions of arginine-rich histones exhibited distinct differences in their capacity to block DNA accessibility.
  • Phosphorylation of lysine-rich histones decreased their blocking efficacy against DNase activity.

Cite This Study

Mirsky et al. (1972) studied this question.

synapsesocial.com/papers/6a911fc2cd6c08c0eacaf390https://doi.org/10.1073/pnas.69.11.3243
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Blocking by Histones of Accessibility to DNA in Chromatin1972 · 36 citations
  2. 2The Structure of Chromatin1971 · 71 citations
  3. 3HISTONES WITH HIGH LYSINE CONTENT1955 · 96 citations
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  5. 5ACTIVE AND INACTIVE REGIONS OF NUCLEAR CHROMATIN AS REVEALED BY ELECTRON MICROSCOPE AUTORADIOGRAPHY1964 · 248 citations