Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin

Three strains, KV-810(T), KV-811 and KV-816, were isolated from mangrove soil from a southern island in Japan on media supplemented with ascorbic acid or rutin. These strains contained l-ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan and DMK-9(H(4)) as the predominant menaquinone. The G+C content of the DNA was 70-72 mol%. These characteristics in combination with 16S rRNA gene sequence analysis revealed that the novel strains belonged to the genus Demequina. The DNA-DNA hybridization values showed that the three new strains belonged to the same species, a novel species of the genus Demequina. Therefore strains KV-810(T), KV-811 and KV-816 are proposed as representing a novel species, Demequina salsinemoris sp. nov. The type strain is KV-810(T) (=DSM 22060(T)=NBRC 105323(T)).