Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
February 23, 2023Frontiers in Plant ScienceOpen Access

Genome-wide identification of DnaJ gene family in Catalpa bungei and functional analysis of CbuDnaJ49 in leaf color formation

View Full Paper
Ask AI
Bookmark
Share

Authors

YYYingying YangLZLinjiao ZhaoJWJunhui Wang

Discussion

Loading...

Member takes

Overview

Genome-wide functional study reveals DnaJ49-mediated regulation of leaf pigmentation in Catalpa bungei, indicating a novel target for ornamental plant breeding.

Key Points

  • Identify the DnaJ gene family in Catalpa bungei and determine the functional role of CbuDnaJ49 in regulating leaf color.
  • Conducted genome-wide identification, domain classification, gene structure, collinearity, and promoter analyses of the DnaJ family in Catalpa bungei.
  • Profiled differential transcriptomes between green and yellow leaf sectors of Catalpa bungei cultivar 'Maiyuanjinqiu' to detect candidate color-regulating genes.
  • Performed ectopic overexpression of candidate gene CbuDnaJ49 in tobacco seedlings and measured pigment levels relative to wild-type controls.
  • Identified 88 putative CbuDnaJ genes categorized into four structural types, showing evidence of tandem and segmental duplications during evolutionary expansion.
  • Identified CbuDnaJ49 as the most prominently differentially expressed DnaJ family member between yellow and green leaf sectors.
  • Overexpression of CbuDnaJ49 in transgenic tobacco generated an albino leaf phenotype marked by significant reductions in both chlorophyll and carotenoid concentrations compared to wild type.

Cite This Study

Yang et al. (2023) studied this question.

synapsesocial.com/papers/6a913d6ca97dfa43b74638fdhttps://doi.org/10.3389/fpls.2023.1116063
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain Gene in albostrians Barley2019 · 82 citations
  2. 2Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling2018 · 115 citations
  3. 3Loss of GLK1 transcription factor function reveals new insights in chlorophyll biosynthesis and chloroplast development2019 · 145 citations
  4. 4Chloroplast DnaJ-like proteins 3 and 4 (CDJ3/4) from Chlamydomonas reinhardtii contain redox-active Fe–S clusters and interact with stromal HSP70B2010 · 32 citations
  5. 5Pea Chloroplast DnaJ-J8 and Toc12 Are Encoded by the Same Gene and Localized in the Stroma2010 · 35 citations