Pyridoxal dehydrogenase was partially purified from extracts of Pseudomonas sp.MA grown on pyridoxamine as the sole carbon and nitrogen source, and shown to catalyze Reaction i. Pyridoxal (hemiacetal)f DPN+ + (i) 4-pyridoxolactone + DPNH + H+Pyridoxal dehydrogenase is specific for DPNf and does not oxidize benzaldehyde, acetaldehyde, salicylaldehyde, or Sdeoxypyridoxal.In the presence of the latter compound, oxidation of pyridoxal is stimulated, apparently because of the occurrence of the dismutation reaction (ii).
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Burg et al. (1969) studied this question.
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