A protoheme derivative with covalently-bound tris(2,2′-bipyridine) ruthenium (II) [Ru(bpy)3]2+ was successfully incorporated into apo-myoglobin. In the reconstituted myoglobin emission of *[Ru(bpy)3]2+ was quenched intramolecularly, the efficiency being greater by more than 30-fold than that of the corresponding intermolecular process.
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Hamachi et al. (1993) studied this question.
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