Sequence of Bovine Liver Glutamate Dehydrogenase

A mixture containing predominantly two large peptides, CNBr2 and CNBr10, from carboxymethylated glutamate dehydrogenase of bovine liver was investigated by studying two types of enzymic hydrolysates. In the first case, the mixture was maleylated and hydrolyzed with trypsin and the resulting peptides were isolated. In the second study 14C was present in the carboxymethyl groups. The mixture was hydrolyzed with thermolysin and the resulting peptides were isolated. From these studies it was possible to provide a number of important overlapping sequences, to confirm the sequence in certain critical areas, and to conclude that no sequence containing a cysteine residue was missing. From the results it appears likely that no part of the sequence of the enzyme is missing.