Why the study?
Is Quantitative Real-Time PCR more sensitive than conventional PCR?
Is Quantitative Real-Time PCR more sensitive than conventional PCR?
The text discusses whether quantitative real-time PCR offers greater sensitivity than conventional PCR in infectious disease diagnostics.
qPCR sensitivity advantage over conventional PCR remains unproven in infectious diagnostics; leaves open standardized comparative trials before clinical adoption.
Molecular methods, essentially based upon PCR, have become an indispensable tool in the diagnosis of infectious diseases. Real-time quantitative PCR (qrtPCR), as a novel technology, has revolutionized molecular diagnostics by adding reliability and speed ([15][1], [28][2]). However, Apfalter et al
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Bastien et al. (2008) studied this question.
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