A gene library of Mycobacterium bovis DNA in lambda gt11 was screened for the expression of M. bovis proteins using monoclonal antibodies and the streptavidin-biotinylated peroxidase detection system. Analysis of several phage that gave positive signals revealed that identical results could be obtained in the absence of specific antibody. These recombinants appear to encode biotinylated M. bovis proteins. A similar result was obtained with independently constructed libraries of M. bovis BCG and M. leprae.
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Collins et al. (1987) studied this question.
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