A previously described polymerase chain reaction (PCR) assay results in the amplification of a 188-bp product from Gaeumannomyces graminnis var. graminis fungal template DNA derived from boiled fungal mycelia. Similar results are obtained when mycelia are grown on potato-dextrose agar, Czapek solution agar, and Luria-Bertani agar, but not on a medium selective for Gaeumannomyces (SM-7). Amplification of this specific DNA fragment combined with observation of lobed hyphopodia can be used to identify a Gaeumannomyces-like isolate as G. g. graminis, even if perithecia are never produced by the isolate. All 40 «presumptive» G. g. graminis test isolates produced lobed hyphopodia in culture and the 188-bp PCR product, but only 19 of these isolates produced mature perithecia []
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Monica L. Elliott (1993) studied this question.