A cell-free system has been reported for the biosynthesis of nicotianamine, a putative precursor of mugineic acid and its derivatives (MAs). L-[l-14C]methionine was incubated with a crude homogenate of iron-deficient barley roots, and the reaction products were analyzed by HPLC and TLC. Radioactive nicotianamine was formed enzymatically in a reaction that was dependent on ATP. The effect of aminooxyacetic acid (AOA) was compared between systems for the synthesis of nicotianamine in vivo and in vitro.
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Shojima et al. (1989) studied this question.