Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes

A new biotin derivative functionalized by a pyrrole group has been synthesized. This functionalization has allowed the preparation of the first example of a poly(pyrrole-biotin) film by oxidative polymerization. In addition, the successful copolymerization of a polypyridinyl complex of ruthenium(II) containing pyrrole groups with the biotin derivative provided redox copolymers exhibiting specific adsorption sites. The resulting copolymer electrodes were applied to the immobilization of a biotinylated glucose oxidase through an avidin bridge. An avidin-conjugated alkaline phosphatase was also immobilized by direct specific adsorption onto the biotinylated polymer. The amperometric response of the resulting biosensors to glucose and phenylphosphate has been studied.