A quartz crystal microbalance (QCM) bioaffinity sensor for avidin, based on electropolymerized biotinylated polypyrrole film, was developed. The functionalization of one of the Au quartz electrode has been achieved by controlled potential oxidation of a biotinyl pyrrole monomer in CH3CN. The modified quartz resonator was placed in a QCM flow cell. In the presence of avidin solutions, gravimetric measurements indicated the reproducible formation of a compact avidin layer via the specific avidin-polymerized biotin interaction. The polymerized biotin retained identical bioaffinity properties for avidin over a period of one month. The initial slope of the frequency change as a function of time was recorded and related to avidin concentrations. Avidin concentration in the range 1.5×10−9–3.7×10−5 M can be measured by this method.
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Cosnier et al. (2001) studied this question.
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