The immobilization of enzymes such as cellulase by radiation polymerization of dispersed polyurethane prepolymer was studied using tolylene‐2,4‐diisocyanate and 2‐hydroxyethyl methacrylate. The polyurethane particles were obtained by the dispersion of polyurethane prepolymer followed by radiation polymerization, in which the enzyme was immobilized on its surface by convalent bonding. The particle diameter of immobilized enzyme particles varied with monomer concentration and composition. The enzymatic activity of immobilized enzyme particles varied with the temperature of dispersion and irradiation, and decreased with increasing particle diameter.
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Kumakura et al. (1983) studied this question.
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