Evidence for the localization of extracellular pectinesterases was obtained in hyphae of Phytophthora infestans with the antibody – protein A – gold technique. Hyphae were fixed in 1% formaldehyde and 0.5% glutaraldehyde, and the immunocytochemical localization was done on ultrathin sections of tissue embedded at low temperature in Lowicryl K4M. Gold particles were mainly present over three different types of vesicles and over dictyosomes. In older parts of the hyphae, the plasma membrane was heavily labeled. This might suggest that the hyphal cell wall in subapical regions is not as permeable as in the hyphal tips.
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Förster et al. (1987) studied this question.
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