3α, 7α, 12α-Trihydroxy-5β-cholestane-24-carboxylic acid was synthesized. 2. It was verified synthetically that trihydroxybufosterocholenic acid isolated from toad bile is 3α, 7α, 12α-trihydroxy-5β-cholest-22-ene-24-carboxylic acid.
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Hoshita et al. (1967) studied this question.