Ab initio structure determination from experimental fluctuation X-ray scattering data

This is the authors' abstract. We don't add key points for this paper.

Significance Fluctuation X-ray scattering is a biophysical structural characterization technique that overcomes low data-to-parameter ratios encountered in traditional X-ray methods used for studying noncrystalline samples. By collecting a series of ultrashort X-ray exposures on an ensemble of particles at a free-electron laser, information-dense experimental data can be extracted that ultimately result in structures with a greater level of detail than can be obtained using traditional X-ray scattering methods. In this article we demonstrate the practical feasibility of this technique by introducing data-processing techniques and advanced noise-filtering methods that reduce the required data collection time to less than a few minutes. This will ultimately allow one to visualize details of structural dynamics that may be inaccessible through traditional methods.