Antigens were extracted with EDTA from 32 strains representing 10 species of Clostridium. When these antigens were examined by an enzyme-linked immunosorbent assay, marked cross-reactions were observed between C. difficile, C. sordellii and C. bifermentans. The cross-reactive antigen, visualized by crossed immunoelectrophoresis, was carbohydrate.
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Poxton et al. (1981) studied this question.
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