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January 1, 1994Applied and Environmental MicrobiologyOpen Access

Use of solid-phase PCR for enhanced detection of airborne microorganisms

Abdiel J. Alvarez Mark P. Buttner Gary A. Toranzos Elizabeth A. Dvorsky Ana Lucia Henao Toro T B Heikes L E Mertikas-Pifer Linda D. Stetzenbach

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Abstract

The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.

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Cite This Study

Alvarez et al. (1994) studied this question.

synapsesocial.com/papers/6a918e73cdcb4f7bdda15e58 https://doi.org/10.1128/aem.60.1.374-376.1994

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