As little as 5 micrograms of interstitial cell stimulating hormone (ICSH) or 20 micrograms of ICSH-beta is effective for the induction of ovulation in 100 percent of hamsters treated at 0500 hours on day 4 after lordosis, whereas as much as 800 micrograms of ICSH-alpha is ineffective. Both ICSH and ICSH-beta are also effective for induction of ovulation in hypophysectomized animals. Thus, the ovulation-inducing activity of the ICSH molecule resides in its beta subunit.
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Yang et al. (1972) studied this question.
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