The structure of HVJ. II. The fine structure of the subunits.

The Structure of HVJ* 11.The Fine Structure of the Subunits YAsuHiRO HosAKA, YosniMi NisHi AND KONosuKE FUKAi h was 10/11td Ihat cihcr disii, tern ation or I{VJ, in the I, rusencc or Emasol, in the cold is an effective inclhocl for' the preparatioil or aiT RNA containing fraction as well as Ihc hcmaggluiinin fraciion or Ihc \, Iru*.Tllc line siriicturc or the fractions thus obtainecl \\, as studiecl by the Ilcgativc staining inclhodThe bulk or the \, it al RXi\ \\, as present in the fraction or the ifeaicd HVJ sedimenting at 12,000ipm (17,000 g).TITis11'action ITad an RNA content or 2.0 -2.5 per cent, whereas Ihc RNA conteitL of the original \, irus particles was 0.75-1.0put cent, and 11 showed a SPCcilic gel cliffusion ai, d setlimcntatioil paltcrn allhougl, contaminated with a small amount of hemaggluiinin TIT negatively stained prcparatioits the \, Irus particles of HVJ hatl vaiious shapes and SIzcs or 1500 -6000 A width.The particles ITacl a surfacc layer, 60 -90 A thick with projections of 130 -150 A ICngtlt and 10 -20 A \\, idih.irisidc the surface layer, inner strandcd materials wetc seen Thc hemagglutinin fraction ITas I, articles or 450 -550 A \\, idth and greatcr length with ap-proximateIy 500 A , will, pro^Ciions similar' 10 111DSC o1T Ihc surface of Ihc intact \, irus pariiclesThese pailiclcs seemed to form the outer envelope of the intact virus particles by conncct willt cacll o1heiThe RNA coniaiiting11'action consisted mainly oldoublellclicalsiructurcs o1175 -190 A \\. Idth and varying Iengtll ancl seemccl to Form Ihc inner structure or Ihc intact \, irus particles.The non-hemagglutinii, ITactioi, consisicrl of maifj, loosened or unwound helical strands, possibly derived from the original hclical situcturc of the RNA-coniaining fraction.anti Furihci or the clotiblc helical SII'ucturcs aitd litc in activatecl hemaggluiininsThe significance or these findings \\, as cliscLissccl