To study directly the effects of hypoxia on the mammalian embryo, rat embryos at the 14‐, 22‐, and 35‐somite stages were explanted into the in vitro system of New. The initial heart rates in the three groups did not differ. With hypoxia the 22‐ and 35‐somite embryos responded with a prompt heart‐rate drop but no significant effect was found in the 14‐somite embryos. Irreversible sludging of yolksac circulation was associated with death in the older embryos.
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Shepard et al. (1969) studied this question.
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