A rapid, simple, and sensitive procedure for the determination of theophylline in plasma using second derivative spectroscopy is described. Second derivative spectra of plasma extracts containing theophylline in concentrations ranging from 2 to 20 micrograms/mL were studied. The method is suitable for the measurement of plasma theophylline in patients treated for cardiorespiratory disorders. Interference from other compounds such as phenobarbital can be avoided.
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Bermejo et al. (1985) studied this question.
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