March 1, 1986Journal of Biological ChemistryOpen Access

Solubilization and hydrodynamic characterization of the dihydropyridine receptor from rat ventricular muscle.

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Population

Rat heart membranes (preclinical model)

Design

Preclinical

Authors

WHWilliam A. HorneMichigan State University GWGregory A. WeilandNational Institutes of Health RORobert E. OswaldCornell University

Discussion

Member takes

Implication

Characterizes the dihydropyridine receptor complex in rat ventricle; leaves open translation to human calcium channel biology.

Structured PICO

Population

Rat heart membranes (preclinical model)

Intervention

Solubilization with a detergent mixture of digitonin and Triton X-100, followed by hydrodynamic characterization in Tween 80, CHAPS, and digitonin

Outcome

Hydrodynamic properties including S20,w values, Stokes radius, partial specific volume, and molecular weightsurrogate

The study successfully solubilized and characterized the hydrodynamic properties of the dihydropyridine receptor-calcium channel complex from rat ventricular muscle, estimating its molecular weight at 370,000.

Cite This Study

Horne et al. (1986) studied this question.

synapsesocial.com/papers/6a923fbe3eb7c78f2e41de9e https://doi.org/10.1016/s0021-9258(17)35687-9

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Solubilization and hydrodynamic characterization of the dihydropyridine receptor from rat ventricular muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Solubilization and hydrodynamic characterization of the dihydropyridine receptor from rat ventricular muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider