FIG 1 Analysis of porin expression during MIC analysis.(A) Membrane proteins were isolated by Sarkosyl extraction, and 10 g was separated on 8 to 12% bis-Tris acrylamide gels prior to Coomassie staining.Lane 1, ¢9; lane 2, ¢9⌬PorA; lane 3, ¢9⌬PorB.(B) Samples from the last well showing turbidity were plated on BHI agar and immunoblotted with the PorA-specific MAb MN14C11.6.
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Peak et al. (2013) studied this question.
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