Direct electron transfer between chloroperoxidase (CPO) from Caldariomyces fumago and gold electrode has been achieved by layer-by-layer adsorption of CPO and polydimethyldiallylammonium chloride (PDDA) monolayers on a gold electrode. QCM frequency shift demonstrated monolayer formed during each adsorption. Electrochemical characterization studies show thin layer properties of these PDDA–CPO–PDDA films. The PDDA–CPO–PDDA ultra-thin films were stable, up to 97% electrochemical activity remained after two days storage in phosphate buffer pH 5.
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Lu et al. (2007) studied this question.
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