Why the study?
Does cytospin-enhanced direct immunofluorescence (DFA) accurately detect human metapneumovirus compared to RT-PCR in clinical samples?
Does cytospin-enhanced direct immunofluorescence (DFA) accurately detect human metapneumovirus compared to RT-PCR in clinical samples?
The Light Diagnostics HMPV DFA reagent shows reasonable sensitivity compared to RT-PCR, offering a commercially available option for clinical laboratories.
DFA may support rapid HMPV detection but should not yet change practice; leaves open need for prospective validation against RT-PCR.
A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.
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Landry et al. (2008) studied this question.
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