Three basic protocols are provided in this unit, corresponding to the major applications of gel filtration. One method describes desalting or group separation, in which the target protein and contaminating solutes differ substantially in molecular size. Another is used for protein fractionation, application is in characterizing the molecular dimensions of proteins. A Support Protocol is also provided for calibrating gel filtration columns to be used in estimating molecular size.
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Lars Hagel (1998) studied this question.
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