Ursolic Acid Induces Neomyogenesis Through Hyperplasia and Increasing of Satellite Cells Number in Mice Skeletal Muscle

Introduction: Ursolic Acid (UA) is the main compound present in apple cuticular wax as well as in the leaves. Hypertrophic effects of UA on the skeletal muscle ventured us to investigate if UA might activate satellite cells proliferation, and also generate new muscle fibers is so-called ” hyperplasia”.Methods: To confirm this hypothesis, UA administrated twice daily for 7 days to 8 weeks old mice (C57BL/6) and then skeletal muscle tissues isolated for new muscle fibers and satellite cells determination by histochemical and immunohistochemical tests, respectively.Results: Our results demonstrated that UA contributed to skeletal muscle rejuvenation through increasing in the satellite cells number and stimulation of these edge cells to new muscle fiber generation.Conclusion: Taken together, to the best of our knowledge, current findings sharpen our understanding of UA might be as an improvement of skeletal muscle performance and suggest that UA as potential pharmacological purpose to treat the major muscular atrophy diseases such as skeletal muscle atrophy.