The use of calcium alginate gels for “solids separation” and “diffusional chromatography” of biological materials

The use of calcium alginate gels for the “Solids Separation” techniques was demonstrated by entrapment of a yeast extract in calcium alginate pellets and the study of the release of alcohol dehydrogenase and NADH into a dilute calcium chloride solution. The use of calcium alginate gels for a “Diffusional Chromatography” technique was demonstrated in a model system by a fractionation of NAD and hemoglobin release following their entrapment in calcium alginate pellets. The advantages of these techniques and their potentials are discussed.