What question did this study set out to answer?

To evaluate the inhibitory effect of lactate on proline oxidase and determine how this interaction influences metabolic fuel allocation and hyperprolinemia.

December 1, 1977Proceedings of the National Academy of SciencesOpen Access

Regulation of proline oxidase activity by lactate.

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Authors

EKE.M. KowaloffHoward Hughes Medical Institute JPJames M. PhangFrederick National Laboratory for Cancer Research AGAlnora S. Granger

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Implication

Biochemical study demonstrates that lactate inhibits proline oxidase in metabolic pathways, suggesting a mechanism for fuel allocation and hyperprolinemia in lactic acidosis.

Key Points

To evaluate the inhibitory effect of lactate on proline oxidase and determine how this interaction influences metabolic fuel allocation and hyperprolinemia.
Measured proline oxidase enzyme activity and kinetics under varying concentrations of lactate in vitro.
Lactate inhibits proline oxidase, with the enzyme's Km for proline progressively increasing at higher lactate concentrations.
Pronounced inhibition occurs at lactate concentrations typical of clinical lactic acidosis, identifying an enzymatic basis for secondary hyperprolinemia.

Cite This Study

Kowaloff et al. (1977) studied this question.

synapsesocial.com/papers/6a92ae96e55f7294c9979a49 https://doi.org/10.1073/pnas.74.12.5368

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Implication

Biochemical study demonstrates that lactate inhibits proline oxidase in metabolic pathways, suggesting a mechanism for fuel allocation and hyperprolinemia in lactic acidosis.

Regulation of proline oxidase activity by lactate.

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of proline oxidase activity by lactate.

Discussion

Member takes

Implication

Key Points

Cite This Study

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