The relationship between the concentration of nine organic acid substrates (lactic, pyruvic, fumaric, malic, acetic, succinic, glycolic, citric, and formic) and their velocities of uptake has been investigated in West Blue Lake, Manitoba. Maximum uptake velocities were ranked and compared with bacterial plate counts on minimal media supplemented with single organic acids. For pyruvic, fumaric, malic, acetic, glycolic, and citric acids an excellent correlation (r = 0.98) existed between plate counts and acid uptake indicating that for these substrates maximum velocities of uptake are indeed indicative of actual bacterial populations.
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Robinson et al. (1973) studied this question.
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