Effect of Genistein on Antioxidative Defence System and Membrane Fluidity in Chick Skeletal Muscle Cells

This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40, and 80 mol/L genistein in 50 mol/L FeSO 4 / H 2 O 2 treated cells for 24 h. Genistein supplementation recovered the decreased activity of total superoxide dismutase induced by FeSO 4 / H 2 O 2 , significantly increased glutathione peroxidase activity (p<0.05) and decreased malondialdehyde production (p<0.05). The treatment of 80 mol/L genistein in FeSO 4 /H 2 O 2 treated cells decreased the secretion of creatine kinase (p<0.05). Fluorescence polarization values and microviscosities observed with FeSO 4 /H 2 O 2 treated cells were significantly higher than those observed with no FeSO 4 /H 2 O 2 treated cells. The addition of 80 mol/L genistein improved the increased fluorescence polarization value (p<0.05) caused by FeSO 4 /H 2 O 2 treatment. The microviscosity value was significantly decreased by adding genistein (p<0.05). In conclusion, genistein protected skeletal muscle cells from oxidative damage by improving antioxidative status and membrane fluidity.