Key result
Trypsin activation of rat plasma generates an interfering tetradecapeptide-like material from angiotensinogen, which must be removed by cation-exchange chromatography to accurately measure inactive renin.
Identifies a methodological artifact in measuring inactive renin in rat plasma and provides a purification step to enable accurate routine assays.
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Artifact-prone inactive renin assays in rats warrant methodological caution; leaves open improved accuracy for future preclinical renin studies.
Johannessen et al. (1989) studied this question. Trypsin activation was evaluated on Generation of Ang I immunoreactive material and measurement of inactive renin. Trypsin activation of rat plasma generates an interfering tetradecapeptide-like material from angiotensinogen, which must be removed by cation-exchange chromatography to accurately measure inactive renin.
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