Liquid Chromatographic Retention Behavior of Polycyclic Aromatic Hydrocarbons on Newly-Synthesized Chitosan Stationary Phases Cross-Linked With Long Aliphatic Chains

Novel cross-linked chitosan materials have been introduced as the stationary phase in microcolumn liquid chromatography (micro-LC). Three types of cross-linked chitosan phases were synthesized with changing the degree of cross-linking, and the retention behavior for polycyclic aromatic hydrocarbons (PAHs) was compared with that obtained by various commercially available stationary phases, including monomeric and polymeric octadecylsilicas (ODSs) and chemically modified chitosan phases. The results clearly showed the applicability of the newly-synthesized cross-linked chitosan phases as the stationary phase in LC. From the considerations of the shape selectivity, it was also demonstrated that the cross-linked chitosan phase, synthesized with a higher degree of cross-linking reaction, possessed a strong molecular planarity recognition power over typical polymeric ODS phase.