Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats’ colon via NF-κB pathway

// Guanqun Chao 1 and Shuo Zhang 2 1 Department of Family Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China 2 Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China Correspondence to: Shuo Zhang, email: zhangshuotcm@163.com Keywords: irritable bowel syndrome, aquaporins, NF-κB Received: November 04, 2016 Accepted: April 18, 2017 Published: May 02, 2017 ABSTRACT Objective: Our research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models’ colon so as to find novel pathogenesisof IBS. Results: The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group ( p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group ( p < 0.05). Materials and Methods: 18 adult female SD big rats were divided into three groups:the rats in control group were normal rats,the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8. Conclusions: Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.