Aminopropylsilane treatment for the surface of porous glasses suitable for enzyme immobilisation

During silylation of porous glass with 3-aminopropyltriethoxysilane, the properties of the carrier affected the concentration of bound amino groups, the formation of aminopolysiloxane mono- or multilayer on the surface, and the hydrolytic stability of the layer formed. The influence of the carrier depended on the specific surface area and the size of pores. In contrast to silylation performed in organic solvents, in aqueous solutions a monolayer of aminopolysiloxane of high hydrolytic stability was formed on the surface of porous glass. Tetraethoxysilane modification of porous glass prior to silylation with aminosilane yields carriers of increased hydrolytic stability. Glucoamylase immobilised on carriers, that were modified in aqueous solutions, exhibit higher enzymatic activity.