Murine monoclonal antibodies binding to the specific antigens of aspergillus fumigatus associated with allergic bronchopulmonary aspergillosis

Four murine monoclonal antibodies, which were produced against Aspergillus fumigatus antigens using hybridoma technology, reacted with different antigenic components of A. fumigatus, and in turn these antigens showed reactivity with the sera from allergic bronchopulmonary aspergillosis (ABPA). All four antibodies were of IgM isotype. These antibodies reacted against eight antigen preparations from three different strains of A. fumigatus by enzyme-linked immunosorbent assay (ELISA). Only two of four antibodies reacted with the antigens in crossed immunoelectrophoresis (CIE), rocket immunoelectrophoresis, and agar gel double diffusion. In western blot analysis it was found that the antigenic components reacting with the monoclonal antibodies were mostly of the low molecular weight components of A. fumigatus antigens. These components also showed binding to both IgG and IgE antibodies in the sera of ABPA patients, but failed to show any reactivity with sera from aspergilloma patients. Hence these antigenic components may be of diagnostic significance and can be isolated using immunoaffinity chromatography.