Differentiation of smoked Salmo salar, Oncorhynchus mykiss and Brama raii using the nuclear marker 5S rDNA

PCR amplification of 5S rDNA was applied to differentiate smoked samples of Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and bream (Brama raii). Contiguous divergent primers for the conserved coding region of the 5S rRNA gene allowed species-specific amplification of tandemly arranged units of the 5S rDNA in these three related species. Thus, the 5S rDNA genetic marker may become very useful for inspection programmes intended to assess the species identity of smoked products.