The results with 0.88 11 sucrose solution as the dispersion medium agree essentially with those obtained with alkaline water medium. 2. Succinoxidase activity of the mitochondria accounts for 43% of the total homogenate activity and cytochrome oxidase 47% on a wet weight basis. 3. On nitrogen basis the activities are 2-3 times greater than that of the homogenate. 4. After 2 washings the activity of both enzymes on a wet weight basis is reduced; on a nitrogen basis succinoxidase activity is reduced to 33% but the cytochrome oxidase activity is the same as in the original mitochondria. 5. Small amounts of the final supernatant fluid added to mitochondria increase the succinoxidase activity to 85% of the original homogenate. This effect does not represent an addition of the activities of the 2 fractions. 6. There is no change in intracellular succinoxidase and cytochrome oxidase activities after a period of starvation of 16 to 140 hours.
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Kretchmer et al. (1953) studied this question.