Preparation and characterization of poly(dimethyldiallyl ammonium)chloride and antiglobulin tests for antibody detection

Poly(dimethyldiallyl ammonium)chloride [poly(DMDAAC)] was prepared in our laboratory and monomer‐to‐polymer structures were characterized by infrared (IR), proton, and carbon‐13 nuclear magnetic resonance (NMR) as well as DEPT techniques. The polyelectrolytes were used to detect the antibodies of red blood cells (RBCs) in human sera and the results were compared with hexadimethrine bromide (polybrene), bromeline, and other methods. This article presents data supporting the use of the manual poly(DMDAAC) test; the 1‐min procedure at room temperature provided a rapid and sensitive test. The states of agglutination of RBCs were investigated by transmission electron microscopy (TEM). The results of the morphology were in agreement with the manual poly(DMDAAC) test.