Alkali-treated hematoporphyrin derivative (Hpd) was separated in four main components by means of high-pressure liquid chromatography. The photosensitizing efficiencies of the components were compared by studying the photosensitized inactivation of human cells of the line NHIK 3025. Photoinduced membrane damage was studied by measuring the leakage of [ 5l Cr]chromate from the cells. The photosensitizing efficiency of the Hpd-components increased with decreasing polarity. This is probably due to a corresponding increase in the cellular uptake of the components. The photosensitizing effect of Hpd is almost entirely due to the least polar one of its main components. This component which contains minor subfractions, is also present in commercially available hematoporphyrin (Hp) and plays the major role for the photosensitizing effect of Hp in our cellular system. For all four components irradiation doses giving about 50% cell inactivation (survival) were required before any leakage of [ 5l Cr]chromate larger than that of the control was observed. Absorption spectroscopy and gel permeation chromatography showed that Hpd in neutral aqueous solutions mainly contained aggregated porphyrins. The least polar components showed the strongest tendency to aggregate.
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Moan et al. (1982) studied this question.
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