A method has been developed for the separation and identification of eugenol in the alcohol extract of cloves directly without having to previously separate other components. Extraction of eugenol with alcohol is followed by analysis with high‐performance liquid chromatography. Separation by reversed‐phase chromatography on low‐polarity μBondapak C18 was achieved with isocratic elution. Tentative identification is based on chromatographic appearance of a peak with retention time 5.54 min for pure standard reference eugenol. The identification of eugenol was confirmed by gas chromatography/mass spectrometry analysis.
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Myint et al. (1995) studied this question.
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